2.3.1.232: methanol O-anthraniloyltransferase

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For detailed information about methanol O-anthraniloyltransferase, go to the full flat file.

Reaction

Synonyms

AMAT, anthraniloyl-coenzyme A (CoA):methanol acyltransferase, anthraniloyl-coenzyme A:methanol acyltransferase

ECTree

2 Transferases

2.3 Acyltransferases

2.3.1 Transferring groups other than aminoacyl groups

2.3.1.232 methanol O-anthraniloyltransferase

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Results	in table
18	AA Sequence
1	Application
1	Cloned(Commentary)
2	Inhibitors
4	kcat/KM [mM/s]
4	KM Value [mM]
4	Metals/Ions
2	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
2	Organism
1	pH Optimum
1	pH Stability
1	Purification (Commentary)
1	Reaction
2	Reference
2	Source Tissue
2	Substrates and Products (Substrate)
2	Subunits
4	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Temperature Stability [°C]
4	Turnover Number [1/s]

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Application on EC 2.3.1.232 - methanol O-anthraniloyltransferase

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synthesis

Vitis labrusca

Q3ZPN4

three anthranilate derivatives, N-methylanthranilate, methyl anthranilate, and methyl N-methylanthranilate are synthesized using metabolically engineered stains of Escherichia coli. NMT encoding N-methyltransferase from Ruta graveolens, AMAT encoding anthraniloyl-coenzyme A (CoA):methanol acyltransferase from Vitis labrusca, and pqsA encoding anthranilate coenzyme A ligase from Pseudomonas aeruginosa are cloned and Eschetrichia coli strains harboring these genes were used to synthesize the three desired compounds. Escherichia coli mutants (metJ, trpD, tyrR mutants), which provide more anthranilate and/or S-adenosyl methionine, are used to increase the production of the synthesized compounds. 0.1853 mM N-methylanthranilate and 0.0952 mM methyl N-methylanthranilate are synthesized

757422