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2.3.1.217: curcumin synthase

This is an abbreviated version!
For detailed information about curcumin synthase, go to the full flat file.

Word Map on EC 2.3.1.217

Reaction

feruloyl-CoA
+
feruloylacetyl-CoA
+
H2O
= 2 CoA +
curcumin
+
CO2

Synonyms

curcumin synthase, curcumin synthase 1, curcumin synthase 2, CURS, CURS1, CURS2, CURS3, OsCUS, ZoCURS

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.217 curcumin synthase

Cloned

Cloned on EC 2.3.1.217 - curcumin synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CURS1, DNA and amino acid sequence determination and analysis, expression analysis by quantitative real time PCR
CURS2, DNA and amino acid sequence determination and analysis, expression analysis by quantitative real time PCR
CURS3, DNA and amino acid sequence determination and analysis, expression analysis by quantitative real time PCR
diketide-CoA synthase, curcumin synthase, and the unnatural fusion protein diketide-CoA synthase:CURS His-tagged at their N terminus are produced in Escherichia coli BL21 (DE3)
DNA and amino acid sequence determination and analysis, expression analysis by quantitative real-time PCR, recombinant expression as N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expression in Escherichia coli
expression of His4-tagged CURS1 in Escherichia coli strain BL21(DE3)
PAL (phenylalanine ammonia lyase) or TAL (tyrosine ammonia lyase), along with Os4CL (p-coumaroyl-CoA ligase) and CUS (curcumin synthase) genes, are introduced into Escherichia coli