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2.3.1.164: isopenicillin-N N-acyltransferase

This is an abbreviated version!
For detailed information about isopenicillin-N N-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.164

Reaction

phenylacetyl-CoA
+
isopenicillin N
 +
H2O
=
CoA
 +
penicillin G
 +
L-2-aminohexanedioate

Synonyms

6-aminopenicillanate acyltransferase, 6-aminopenicillanic acid acyltransferase, 6-aminopenicillinanic acid phenylacetyltransferase, 6-aminopenicillinanic acylase, aatA, aatB, acyl coA:isopenicillin N-acyltransferase, acyl coenzyme A: isopenicillin N acyltransferase, acyl coenzyme A:6-aminopenicillanic acid acyltransferase, acyl-CoA:6-aminopenicillanate acyltransferase, acyl-CoA:6-APA acyltransferase, acyl-CoA:isopenicillin N acyltransferase, acyl-coenzyme A: IPN acyltransferase, acyl-coenzyme A:isopenicillin N acyltransferase, acyl-coenzyme A:isopenicillin N-acyltransferase, acyltransferase, 6-aminopenicillanate, IAT, IPN acyltransferase, isopenicillin N acyltransferase, isopenicillin N:acyl CoA acyltransferase, isopenicillin N:acyl-CoA: acyltransferase, isopenicillin-N N-acyltransferase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.164 isopenicillin-N N-acyltransferase

Purification

Purification on EC 2.3.1.164 - isopenicillin-N N-acyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
cell extracts of Penicillium chrysogenum are prepared, Escherichia coli soluble extracts are used for in vitro IAT activity measurement
-
recombinant wild-type enzyme with a C-terminal His-tag is purified in a single step using Ni2+ affinity chelate chromatography. A combination of 5 mM DTT + 250 mM NaCl + 5 mM EDTA is effective in preventing aggregation of the enzyme
-