2.3.1.136: polysialic-acid O-acetyltransferase
This is an abbreviated version!
For detailed information about polysialic-acid O-acetyltransferase, go to the full flat file.
Word Map on EC 2.3.1.136
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2.3.1.136
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retinyl
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retinoids
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esterification
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retinol-binding
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arats
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crbp
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11-cis-retinal
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a-deficient
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acyl-coa:retinol
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cyp26a1
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stra6s
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all-trans-ra
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coa:retinol
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all-trans-retinyl
- 2.3.1.136
-
retinyl
-
retinoids
- esterification
-
retinol-binding
-
arats
- crbp
- 11-cis-retinal
-
a-deficient
-
acyl-coa:retinol
-
cyp26a1
-
stra6s
-
all-trans-ra
-
coa:retinol
-
all-trans-retinyl
Reaction
Synonyms
lecithin:retinol acyltransferase, LRAT, NeuO, OatC, OatWY, polySia specific O-acetyltransferase, polysialic acid O-acetyltransferase, polysialic acid O-AcTase, polysialic acid specific O-acetyltransferase, polysialic acid-specific O-acetyltransferase, polysialic-acid O-acetyltransferase
ECTree
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Engineering
Engineering on EC 2.3.1.136 - polysialic-acid O-acetyltransferase
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H119A
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complete loss of enzymatic activity without affecting the oligomerization state
NeuO + 12 hepta-nucleotide repeats
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a set of NeuO alleles with N-terminal 0, 12, 24, and 36 hepta-nucleotide repeats are generated: protein yield: 3.7 mg/L, Km (mM): 0.22 (acetyl-CoA), 1.1 (colomnic acid), kcat (1/sec): 0.56 (acetyl-CoA), 1.85 (colomnic acid)
NeuO + 24 hepta-nucleotide repeats
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a set of NeuO alleles with N-terminal 0, 12, 24, and 36 hepta-nucleotide repeats are generated: protein yield: 5.0 mg/L, Km (mM): 0.27 (acetyl-CoA), 1.0 (colomnic acid), kcat (1/sec): 1.07 (acetyl-CoA), 2.57 (colomnic acid)
NeuO + 36 hepta-nucleotide repeats
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a set of NeuO alleles with N-terminal 0, 12, 24, and 36 hepta-nucleotide repeats are generated: protein yield: 5.0 mg/L, Km (mM): 0.22 (acetyl-CoA), 0.22 (propionyl-CoA) 1.6 (colomnic acid), kcat (1/sec): 1.16 (acetyl-CoA), 0.09 (propionyl-CoA), 3.84 (colomnic acid)
W143A
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complete loss of enzymatic activity without affecting the oligomerization state
DELTA1-103
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mutant lacking the first 103 amino acids: after purification mutant is found as a monomer, indicating that dimerization of OatC depends on the first 34 amino acids. Mutant shows no activity
DELTA1-34
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mutant lacking the first 34 amino acids: after purification mutant is found as a monomer, indicating that dimerization of OatC depends on the first 34 amino acids. Mutant retains 25% activity
H456A
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mutant shows reduced enzymatic activity, 70% of wild-type level
additional information
Q58WP5
deletion of gene neuO in strain EV291 eliminates enzyme activity, which can be complemented in trans, mutagenesis method, overview, nonfunctional enzyme encoded by inserted gene neuO-4 allele from unequal or mispairing recombination, GenBank AY779019, role of redistribution variant neuO alleles in pathogen evolution, function of localized hypermutability
additional information
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deletion of gene neuO in strain EV291 eliminates enzyme activity, which can be complemented in trans, mutagenesis method, overview, nonfunctional enzyme encoded by inserted gene neuO-4 allele from unequal or mispairing recombination, GenBank AY779019, role of redistribution variant neuO alleles in pathogen evolution, function of localized hypermutability
additional information
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engineering of a neuO gene that allows phase-stable expression of NeuO with four N-terminal heptads
additional information
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deletion of gene neuO in strain EV291 eliminates enzyme activity, which can be complemented in trans, mutagenesis method, overview, nonfunctional enzyme encoded by inserted gene neuO-4 allele from unequal or mispairing recombination, GenBank AY779019, role of redistribution variant neuO alleles in pathogen evolution, function of localized hypermutability
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