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2.3.1.12: dihydrolipoyllysine-residue acetyltransferase

This is an abbreviated version!
For detailed information about dihydrolipoyllysine-residue acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.12

Reaction

acetyl-CoA
+
enzyme N6-(dihydrolipoyl)lysine
=
CoA
 +
enzyme N6-(S-acetyldihydrolipoyl)lysine

Synonyms

acetyltransferase, lipoate, DHLTA, dihydrolipoamide acetyltransferase, dihydrolipoate acetyltransferase, dihydrolipoic transacetylase, dihydrolipoyl acetyl transferase, dihydrolipoyl acetyltransferase, dihydrolipoyl acetyltransferase component E2, dihydrolipoyl acetyltransferase E2p, dihydrolipoyl transacetylase, dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, DLAT, E2, E2p, hE2, Lat1, lipoate acetyltransferase, lipoate transacetylase, lipoic acetyltransferase, lipoic acid acetyltransferase, lipoic transacetylase, lipoylacetyltransferase, More, myelin-proteolipid O-palmitoyltransferase, palmitoyl-CoA:myelin-proteolipid O-palmitoyltransferase, pyruvate dehydrogenase complex component E2, pyruvate dehydrogenase complex component E2p, pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase component, thioltransacetylase A, transacetylase X

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.12 dihydrolipoyllysine-residue acetyltransferase

Cloned

Cloned on EC 2.3.1.12 - dihydrolipoyllysine-residue acetyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a recombinant fragment hL2S (containing the second lipoyl domain (L2), second hinge region, E1-binding domain (S) and third hinge region of hE2, residues 128–330) is overexpressed in Escherichia coli
-
catalytic domain, residues 173-427
-
expressed in Escherichia coli
expressed in Escherichia coli AG1 cells
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
-
expression of subgene for lipoyl domain in Escherichia coli
-
expression of the enzymes' L2 domain as GST-fusion protein
-
functional overexpression of the enzyme E2 with a single hybrid lipoyl domain per subunit, i.e. 1-lip E2, in strain JRG1342 and BL21(DE3), overexpression of the isolated hybrid lipoyl domain in strain JM 101
-
gene pdhC, organized in the pdhCD operon, physical map, DNA and amino acid sequence determination and analysis, expression as His6-tagged enzyme in Escherichia coli
inner lipoyl domain (L2) of dihydrolipoyl acetyltransferase is expressed as a GST fusion protein
-
knock-out mutants
-
overexpression of truncated thrombin-cleavable enzyme in Escherichia coli strain BL21(DE3)
-
the inner lipoyl-bearing domain of dihydrolipoamide transacetylase is expressed as a GST fusion protein in Escherichia coli
-
wild-type and deletion mutants, expression in Escherichia coli
-
wild-type and mutants