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2.1.1.343: 8-amino-8-demethylriboflavin N,N-dimethyltransferase

This is an abbreviated version!
For detailed information about 8-amino-8-demethylriboflavin N,N-dimethyltransferase, go to the full flat file.

Reaction

2 S-adenosyl-L-methionine +

8-amino-8-demethylriboflavin
= 2 S-adenosyl-L-homocysteine +
roseoflavin

Synonyms

N,N-8-amino-8-demethyl-D-riboflavin dimethyltransferase, RosA

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.343 8-amino-8-demethylriboflavin N,N-dimethyltransferase

Purification

Purification on EC 2.1.1.343 - 8-amino-8-demethylriboflavin N,N-dimethyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, phenyl-Sepharose chromatography, anion exchange chromatography, gel filtration on a HiLoad 16/60 Superdex 200 column, analyzed using SDS-PAGE and MS/MS
E7BBN5
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration
E7BBN5
HisTrap column chromatography and MonoQ column chromatography