2.1.1.193: 16S rRNA (uracil1498-N3)-methyltransferase
This is an abbreviated version!
For detailed information about 16S rRNA (uracil1498-N3)-methyltransferase, go to the full flat file.
Reaction
Synonyms
16S rRNA methyltransferase, DUF558 protein, m3U1498 specific methyltransferase, PA0419, RsmE, Rv2372c, YggJ
ECTree
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Substrates Products
Substrates Products on EC 2.1.1.193 - 16S rRNA (uracil1498-N3)-methyltransferase
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REACTION DIAGRAM
S-adenosyl-L-methionine + uracil1498 in 16S rRNA
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
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S-adenosyl-L-methionine + uracil1498 in 16S rRNA
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
activity is dependent on a fully assembled 30 S ribosome subunit
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S-adenosyl-L-methionine + uracil1498 in 16S rRNA
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
small ribosome subunits obtained from an RsmE deletion strain can be methylated by purified RsmE, neither 70S ribosomes nor 50S subunits are active. 16S rRNA obtained from the mutant strain, synthetic 16S rRNA, and 3' minor domain RNA are all very poor or inactive as substrates. 30S particles partially depleted of proteins by treatment with high concentrations of LiCl or in vitro reconstituted intermediate particles also show little or no methyl acceptor activity. RsmE requires a highly structured ribonucleoprotein particle as a substrate for methylation
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S-adenosyl-L-methionine + uracil1498 in 16S rRNA
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
the enzyme is highly specific for U1498 in 16S rRNA. The purified enzyme is able to incorporate methyl groups from S-adenosyl-L-methionine into 30S particles purified from the mutant strain but not into 30S particles purified from wild type. Approximately one methyl group could be incorporated per mutant 30S subunit at high levels of enzyme
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S-adenosyl-L-methionine + uracil1498 in 16S rRNA
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
RsmE requires a highly structured ribonucleoprotein particle (a fully assembled 30S ribosome subunit) as a substrate for methylation, and this methylation occurs late during 30S ribosome assembly
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-
?
S-adenosyl-L-methionine + uracil1498 in 16S rRNA
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
-
-
-
?
S-adenosyl-L-methionine + uracil1498 in 16S rRNA
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
-
-
-
?