1.5.99.B4: flavin-containing opine dehydrogenase
This is an abbreviated version!
For detailed information about flavin-containing opine dehydrogenase, go to the full flat file.
Reaction
Synonyms
BjOpnDH, bll7190, bll7191, bll7192, bll7193, flavin-containing opine dehydrogenase, Nox/Oox-like protein, OdhA, odhB, odhC, ooxA, OpnDH, SoxB
ECTree
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Engineering
Engineering on EC 1.5.99.B4 - flavin-containing opine dehydrogenase
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C382S
Q89E96; Q89E94; Q89E97
site-directed mutagenesis, FAD and FMN are detected at very low level in the extract of the alphaC382Sbetagamma mutant. The recombinant expression level of the alphaC382Sbetagamma mutant is approximately 6fold lower than that of alphabetagamma-wild-type
C61S
Q89E96; Q89E94; Q89E97
site-directed mutagenesis, the alphabetagammaC61S mutant is more resistant to the thermal treatment than the wild-type
C382S
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site-directed mutagenesis, FAD and FMN are detected at very low level in the extract of the alphaC382Sbetagamma mutant. The recombinant expression level of the alphaC382Sbetagamma mutant is approximately 6fold lower than that of alphabetagamma-wild-type
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C61S
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site-directed mutagenesis, the alphabetagammaC61S mutant is more resistant to the thermal treatment than the wild-type
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