1.2.5.3: aerobic carbon monoxide dehydrogenase
This is an abbreviated version!
For detailed information about aerobic carbon monoxide dehydrogenase, go to the full flat file.
Reaction
Synonyms
aerobic Mo/Cu-containing CO dehydrogenase, Carbon monoxide dehydrogenase, CO dehydrogenase, CODH, CoxS, coxSML, CutL, CutM, CutS, EC 1.2.2.4, EC 1.2.3.10, Mo-CODH, Mo-Cu carbon monoxide dehydrogenase, Mo/Cu CODH, MoCu-CODH, molybdenum- and copper-containing carbon monoxide dehydrogenase, molybdenum- and copper-dependent CO dehydrogenase, molybdenum-containing carbon monoxide dehydrogenase, molybdenum-containing CO dehydrogenase, molybdenum-copper carbon monoxide dehydrogenase, molybdenum-copper CO dehydrogenase, molybdenum/copper-containing carbon monoxide dehydrogenase, molybdoenzyme carbon monoxide dehydrogenase
ECTree
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Purification
Purification on EC 1.2.5.3 - aerobic carbon monoxide dehydrogenase
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native enzyme 13fold to homogeneity by ultracentrifugation, anion exchange chromatography, ultrafiltration, hydroxyapatite chromatography, again ultrafiltration, and gel filtration
native enzyme 21fold from cell-free extract by ultracentrifugation, anion exchange chroomatography, ultrafiltration, hydrophobic interaction chromatography, ultrafiltration, and gel filtration
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native enzyme by ammonium sulfate fractionation, dialysis, and anion exchange chromatography, followed by ultrafiltration, and gel filtration
recombinant protein, purification by Ni-NTA and size exclusion chromatography
recombinant recombinant M subunit 8fold by ultracentrifugation, anion exchange chromatography, and gel filtration from Escherichia coli strain BL21(DE3)