1.14.20.1: deacetoxycephalosporin-C synthase
This is an abbreviated version!
For detailed information about deacetoxycephalosporin-C synthase, go to the full flat file.
Word Map on EC 1.14.20.1
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1.14.20.1
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clavuligerus
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chrysogenum
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acremonium
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isopenicillin
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penicillium
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beta-lactams
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epimerase
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cephamycins
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cephalosporium
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ring-expansion
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7-aminodeacetoxycephalosporanic
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pcbab
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ironii
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cephem
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lactamdurans
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synthesis
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7-adca
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2-oxoglutarate-dependent
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cephalexin
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carbenicillin
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doacs
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biotechnology
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medicine
- 1.14.20.1
- clavuligerus
- chrysogenum
- acremonium
- isopenicillin
- penicillium
- beta-lactams
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epimerase
-
cephamycins
- cephalosporium
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ring-expansion
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7-aminodeacetoxycephalosporanic
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pcbab
-
ironii
-
cephem
- lactamdurans
- synthesis
-
7-adca
-
2-oxoglutarate-dependent
- cephalexin
- carbenicillin
-
doacs
- biotechnology
- medicine
Reaction
Synonyms
acDAOC/DACS, cefE, cefEF, Cephalosporin biosynthesis expandase/hydroxylase, DAOC synthase, DAOC/DAC synthase, DAOC/DACS, DAOCS, deacetoxy/deacetylcephalosporin C synthase, deacetoxycephalosporin C synthase, deacetoxycephalosporin-C synthase, deacetoxycephalosporin-C synthetase, deacetoxycephalosporin/deacetylcephalosporin C synthase, expandase, expendase, penicillin N expandase, scDAOCS
ECTree
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Specific Activity
Specific Activity on EC 1.14.20.1 - deacetoxycephalosporin-C synthase
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0.014
purified recombinant wild-type and truncated mutant enzyme, substrate metampicillin
0.023
purified recombinant truncated mutant enzyme, substrate carbenicillin
0.026
0.039
purified recombinant wild-type and truncated mutant enzyme, substrate ampicillin, determined by bioassay
0.071
purified recombinant truncated mutant enzyme, substrate phenethicillin
0.074
purified recombinant wild-type enzyme, substrate phenethicillin
0.133
purified recombinant wild-type enzyme, substrate ampicillin, determined by HPLC
0.156
purified recombinant wild-type enzyme, substrate 6-alpha-methylpenicillin N, substrate conversion
0.178
purified recombinant truncated mutant enzyme, substrate ampicillin, determined by HPLC
0.203
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recombinant enzyme, substrate ampicillin, determined by bioassay
0.237
purified recombinant truncated mutant enzyme, substrate penicillin G
0.24
purified recombinant wild-type enzyme, substrate penicillin G
0.458
recombinant enzyme, substrate ampicillin, determined by bioassay
0.932
11.89
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recombinant mutant S261I, pH not specified in the publication, 30°C
13.44
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recombinant mutant C37S, pH not specified in the publication, 30°C
16.87
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recombinant mutant T42A, pH not specified in the publication, 30°C
160
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recombinant mutant Y184A, pH not specified in the publication, 30°C
2.221
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recombinant enzyme, substrate ampicillin, determined by HPLC
20.03
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recombinant mutant A61E, pH not specified in the publication, 30°C
22.06
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recombinant mutant S261V, pH not specified in the publication, 30°C
30.86
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recombinant mutant S261L, pH not specified in the publication, 30°C
31.38
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recombinant mutant Y184L, pH not specified in the publication, 30°C
33.51
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recombinant mutant Y184M, pH not specified in the publication, 30°C
36.12
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recombinant mutant Y184I, pH not specified in the publication, 30°C
49.12
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recombinant mutant S59T, pH not specified in the publication, 30°C
56.84
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recombinant wild-type enzyme, pH not specified in the publication, 30°C
71.7
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recombinant mutant Q126M, pH not specified in the publication, 30°C
80.68
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recombinant mutant T213V, pH not specified in the publication, 30°C
90.04
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recombinant mutant S261M, pH not specified in the publication, 30°C
98
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recombinant mutant S261A, pH not specified in the publication, 30°C
additional information
purified recombinant wild-type enzyme, substrate carbenicillin
0.932
recombinant enzyme, substrate ampicillin, determined by HPLC
additional information
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activities of wild-type enzyme in ring-expansion and hydroxylation, cosubstrate and substrate conversion rate
additional information
activities of wild-type enzyme in ring-expansion and hydroxylation, cosubstrate and substrate conversion rate
additional information
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relative activities of wild-type and R308 mutants with penicillin variants and analogue substrates, overview
additional information
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activity of recombinant wild-type and mutant enzymes with substrate penicillin G and penicillin N
additional information
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activity with different substrates and assay methods of recombinant wild-type and mutant enzymes, overview
additional information
activity with different substrates and assay methods of recombinant wild-type and mutant enzymes, overview
additional information
160% relative activity with 10 mM substrate penicillin G compared to the activity detected with 1 mM penicillin G
additional information
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160% relative activity with 10 mM substrate penicillin G compared to the activity detected with 1 mM penicillin G
additional information
quaternary mutant (C155Y/Y184H/V275I/C281Y) with enhanced activity and without substrate inhibition
additional information
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quaternary mutant (C155Y/Y184H/V275I/C281Y) with enhanced activity and without substrate inhibition
additional information
the activity of scDAOCS very much depends on the stability of the enzyme maintained by equilibrium in a pool of monomeric and trimeric forms of scDAOCS which can be disrupted by introduction of a relatively small His-tag at the N-terminus of the enzyme
additional information
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the activity of scDAOCS very much depends on the stability of the enzyme maintained by equilibrium in a pool of monomeric and trimeric forms of scDAOCS which can be disrupted by introduction of a relatively small His-tag at the N-terminus of the enzyme
additional information
to achieve optimum conditions for the conversion of penicillin to a cephalosporin, a reaction mixture containing 50 mM Tris-HCl (pH 7.4), 10 mM KCl, 10 mM MgSO4, 0.6 mM ascorbic acid, 0.8 mM ATP, 0.04 mM FeSO4, 0.6 mM 2-oxoglutarate, and 0.28 mM penicillin N is used for detection of the ring-expansion activity of DAOCS from Acremonium chrysogenum, DAOCS is unable to convert penicillin G and other penicillin N analogs under this optimum conditions described for penicillin N
additional information
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to achieve optimum conditions for the conversion of penicillin to a cephalosporin, a reaction mixture containing 50 mM Tris-HCl (pH 7.4), 10 mM KCl, 10 mM MgSO4, 0.6 mM ascorbic acid, 0.8 mM ATP, 0.04 mM FeSO4, 0.6 mM 2-oxoglutarate, and 0.28 mM penicillin N is used for detection of the ring-expansion activity of DAOCS from Acremonium chrysogenum, DAOCS is unable to convert penicillin G and other penicillin N analogs under this optimum conditions described for penicillin N