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1.14.18.6: 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase

This is an abbreviated version!
For detailed information about 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase, go to the full flat file.

Word Map on EC 1.14.18.6

Reaction

a phytoceramide
+ 2 ferrocytochrome b5 +
O2
 + 2 H+ =
a (2'R)-2'-hydroxyphytoceramide
 + 2 ferricytochrome b5 +
H2O

Synonyms

FA2H, AtFAH1, FA2H, FAH1, fatty acid 2-hydroxylase, fatty acid 2-hydroxylase 1, fatty acid alpha-hydroxylase, SCS7, Scs7p, scScs7p, sphingolipid alpha-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.18.6 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase

Purification

Purification on EC 1.14.18.6 - 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
immunoglobulin G-Sepharose 6 resin column chromatography and Superdex 200 gel filtration
recombinant tagged wild-type scScs7p and DELTA95scScs7p from Saccharomyces cerevisiae strain BJ5460 membranes by solubilization with beta-octyl glucoside, ultracentrifugation, affinity/immunoaffinity chromatography, and tag cleavage through rhinovirus 3C protease, which is removed by adding His-Select cobalt resin, followed by ultrafiltration and gel filtration