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1.14.14.91: trans-cinnamate 4-monooxygenase

This is an abbreviated version!
For detailed information about trans-cinnamate 4-monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.91

Reaction

trans-cinnamate
+
[reduced NADPH-hemoprotein reductase]
 +
O2
=
4-hydroxycinnamate
 +
[oxidized NADPH-hemoprotein reductase]
 +
H2O

Synonyms

BnC4H-1, BnC4H-2, C4H, C4H1, C4H2, CA4H, CA4Hase, cinnamate 4-hydroxlase, cinnamate 4-hydroxylase, cinnamate 4-monooxygenase, cinnamate hydroxylase, cinnamate-4-hydroxylase, cinnamic 4-hydroxylase, cinnamic acid 4-hydroxylase, cinnamic acid 4-monooxygenase, cinnamic acid p-hydroxylase, cinnamic-4-hydroxylase, CsC4H, CYP 73, CYP73, CYP73A1, CYP73A10, CYP73A24, CYP73A32, CYP73A5, Cytochrome P450 73, cytochrome P450 cinnamate 4-hydroxylase, EC 1.14.13.11, hydroxylase, cinnamate 4-, IbC4H, LrC4H, More, MpC4H1, MpC4H2, OsaC4H, oxygenase, cinnamate 4-mono-, P450C4H, PaC4H, RsC4H, SbC4H, SmC4H, t-cinnamic acid hydroxylase, trans-cinnamate 4-hydroxylase, trans-cinnamic acid 4-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.91 trans-cinnamate 4-monooxygenase

Cloned

Cloned on EC 1.14.14.91 - trans-cinnamate 4-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
analysis of sequence variants (single nucleotide polymorphism)
cinnamate 4-hydroxylase and cinnamate 4-hydroxylase fused to the FLAG epitope expressed in yeast. A chimeric cinnamate 4-hydroxylase/green fluorescent protein gene is engineered and stable expressed in Arabidopsis
-
expressed in Escherichia coli
-
expressed in Saccharomyces cerevisiae
-
expressed in Saccharomyces cerevisiae strain WAT11
-
expressed in Saccharomyces strain INVSC2(CPR)
expression in hairy roots using Agrobacterium rhizogenes-mediated transformation system
expression in microsomes of yeast strain INVSc1
-
expression in Saccharomyces cerevisiae
expression vector and yeast strain WAT11
gene C4H, DNA and amino acid sequence determination and analysis, expression analysis by quantitative real-time PCR after elicitor treatment
gene SbC4H, DNA and amino acid sequence determination and analysis, quantitative transcription analysis by RT-PCR, sequence comparisons and phylogenetic tree
generation of transgenic tobacco lines with altered activity levels of cinnamic acid 4-hydroxylase by sense or antisense expression of an alfalafa cDNA
-
overexpression in Escherichia coli
quantitative PCR C4H expression analysis in stone fruits and other lignifying tissues, and analysis of involved transcription factors, expression analysis, overview
-
quantitative real-time PCR expression analysis, sequence comparison and phylogenic tree
successful expression requires removal of the intron, yeast does not process the intron at all
-
the binary vector, renamed pBE:100F/101R, containing the coding region of tomato CYP73A24 in the sense orientation under the control of the CaMV35s promoter and nos terminator is transformed into tomato lines Moneymaker, a wild type line, and Old Gold Crimson, a natural high pigment line
-
transformation of Nicotiana tabacum with a truncated enzyme from Phaseolus vulgaris
-
translational fusion with P450 reductase in Escherichia coli
-