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1.14.14.126: beta-amyrin 28-monooxygenase

This is an abbreviated version!
For detailed information about beta-amyrin 28-monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.126

Reaction

beta-amyrin
+ 3 O2 + 3 [reduced NADPH-hemoprotein reductase] =
oleanolate
 + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O

Synonyms

101251676, 101252702, alpha-amyrin 28-hydroxylase, amyrin C-28 oxidase, beta-amyrin 28-hydroxylase, beta-amyrin 28-oxidase, C-28 oxidase, CYP716A12, CYP716A140v2, CYP716A141, CYP716A15, CYP716A17, CYP716A175, CYP716A179, CYP716A244, CYP716A253, CYP716A254, CYP716A44, CYP716A46, CYP716A52v2, CYP716A94, EC 1.14.13.201, lupeol 28-hydroxylase, More, multifunctional oxidase, multifunctional P450, multifunctional triterpene C-28 oxidase, triterpene C-28 oxidase, triterpene monooxygenase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.126 beta-amyrin 28-monooxygenase

Cloned

Cloned on EC 1.14.14.126 - beta-amyrin 28-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
coexpression of PNY1 and CYP716A52v2 in yeast. Transgenic Panax ginseng plants constitutively overexpressing CYP716A52v2 and expressing a CYP716A52v2-RNAi are generated using Agrobacterium tumefaciens mediated genetic transformation
CYP716A244, DNA and amino acid sequence determination and analysis, phylogenetic analysis. quantitative RT-PCR enzyme expression analysis, transcriptomics analysis of plant leaves, recombinant expression in Saccharomyces cerevisiae, recombinant expression in trangsgenic Nicotiana tabacum, co-expression with beta-amyrin synthase, EsBAS
expression in Saccharomyces cerevisiae
gene CYP716A140v2, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR enzyme expression analysis, recombinant coexpression of genes CYP716A140v2 and CYP716A141 in Saccharomyces cerevisiae strain 10, that shows increased cochalic acid levels
gene CYP716A141, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR enzyme expression analysis, recombinant coexpression of genes CYP716A140v2 and CYP716A141 in Saccharomyces cerevisiae strain 10, that shows increased cochalic acid levels
gene CYP716A175, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, transient recombinant expression in Nicotiana benthamiana using the transformation method via Agrobacterium tumefaciens strain GV3101. The higher oxidizing efficiency of CYP716A175 for lupeol and beta-amyrin compared with germanicol preferentially drives the whole reaction chain to the production of betulinate and oleanolate rather than morolic acid. Transient expression in Saccharomyces cerevisiae shows the presence of the intermediate erythrodiol in addition to oleanolate, but not of oleanolic aldehyde. Sequential three-step oxidation reaction leading to oleanolate, low efficiency of CYP716A75 to oxidize erythrodiol in yeast
EB148173
gene CYP716A179, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR enzyme analysis, recombinant expression in Saccharomyces cerevisiae results in production of oleanolic acid, ursolic acid, and betulinic acid from beta-amyrin, alpha-amyrin, and lupeol, respectively, galactose-inducible dual expression of Lotus japonicus CPR1 and CYP716A179
gene CYP716A244, phylogenetic tree, quantitative real-time PCR expression analysis, transient recombinant expression in Nicotiana benthamiana using the transformation method via Agrobacterium tumefaciens strain GV3101, and transient recombinant expression in Saccharomyces cerevisiae with coexpression of Arabidopsis thaliana NADPH-CYP reductase
gene CYP716A253, semi-quantitative and quantitative RT-PCR enzyme expressison analysis, phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain BY4741
gene CYP716A254, DNA and amino acid sequence determination and analysis, phylogenetic tree, heterologous expression in Saccharomyces cerevisiae strain WAT11 results in oxidation of beta-amyrin at the C-28 position to oleanolic acid production
gene CYP716A44, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, in beta-amyrin synthase (EC 5.4.99.39) and CYP716A44 co-expressing Saccharomyces cerevisiae strains SY57 and SY66 erythrodiol and oleanolic acid are detected as C-28 oxidation product of beta-amyrin
gene CYP716A46, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, in beta-amyrin synthase (EC 5.4.99.39) and CYP716A46 co-expressing Saccharomyces cerevisiae strains SY57 and SY66, erythrodiol and oleanolic acid are detected as C-28 oxidation product of beta-amyrin
gene CYP716A94, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain WAT21 microsomes, coexpression with Arabidopsis thaliana NADPH-CYP reductase leads to production of oleanolic acid, mass spectrometric analysis
heterologous expression in Spodoptera frugiperda 9 insect cells and transgenic yeast