1.14.13.167: 4-nitrophenol 4-monooxygenase
This is an abbreviated version!
For detailed information about 4-nitrophenol 4-monooxygenase, go to the full flat file.
Word Map on EC 1.14.13.167
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1.14.13.167
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sumo
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ubiquitin-conjugating
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sumoylation
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hydroxyquinol
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sumo-conjugating
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two-component
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rhodococcus
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ubiquitin-like
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para-nitrophenol
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hydroquinone
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1,2-dioxygenase
- 1.14.13.167
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sumo
-
ubiquitin-conjugating
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sumoylation
- hydroxyquinol
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sumo-conjugating
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two-component
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rhodococcus
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ubiquitin-like
- para-nitrophenol
- hydroquinone
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1,2-dioxygenase
Reaction
Synonyms
2C4NP 4-monooxygenase, 4-NP/4-NCA monooxygenase, NpcA, para-nitrophenol 4-monooxygenase, PdcA, PNP 4-monooxygenase, pnpA, PnpA1
ECTree
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Substrates Products
Substrates Products on EC 1.14.13.167 - 4-nitrophenol 4-monooxygenase
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REACTION DIAGRAM
2-chloro-4-nitrophenol + NADPH + H+ + O2
2-chloro-1,4-benzoquinone + nitrite + NADP+ + H2O
3-methyl-4-nitrophenol + NADPH + H+ + O2
2-methyl-1,4-benzoquinone + nitrite + NADP+ + H2O
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4-nitrocatechol + NADPH + H+ + O2
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lower activity compared to 4-nitrophenol
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4-nitrophenol + NADPH + H+ + O2
1,4-benzoquinone + nitrite + NAD+ + H2O
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?
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17% activity compared to 4-nitrophenol
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2,4,6-trinitrophenol + NADH + H+ + O2
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17% activity compared to 4-nitrophenol
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2-chloro-1,4-benzoquinone + nitrite + NADP+ + H2O
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?
2-chloro-4-nitrophenol + NADPH + H+ + O2
2-chloro-1,4-benzoquinone + nitrite + NADP+ + H2O
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?
2-chloro-4-nitrophenol + NADPH + H+ + O2
2-chloro-1,4-benzoquinone + nitrite + NADP+ + H2O
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?
2-chloro-4-nitrophenol + NADPH + H+ + O2
2-chloro-1,4-benzoquinone + nitrite + NADP+ + H2O
Rhodococcus opacus RKJ300
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?
1,2,4-trihydroxybenzene + nitrite + NAD+ + H2O
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96% activity compared to 4-nitrophenol
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4-nitrocatechol + NADH + H+ + O2
1,2,4-trihydroxybenzene + nitrite + NAD+ + H2O
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96% activity compared to 4-nitrophenol
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1,4-benzoquinone + nitrite + NAD+ + H2O
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4-nitrophenol + NADH + H+ + O2
1,4-benzoquinone + nitrite + NAD+ + H2O
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1,4-benzoquinone + nitrite + NADP+ + H2O
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4-nitrophenol + NADPH + H+ + O2
1,4-benzoquinone + nitrite + NADP+ + H2O
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4-nitrophenol + NADPH + H+ + O2
1,4-benzoquinone + nitrite + NADP+ + H2O
Rhodococcus opacus RKJ300
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4-benzoquinone + nitrite + NADP+ + H2O
monooxygenation
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4-nitrophenol + NADPH + H+ + O2
4-benzoquinone + nitrite + NADP+ + H2O
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the enzyme completely degrades 0.24 mM 4-nitrophenol in 36 min
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no activity with 3-nitrophenol, 2-nitrophenol, 4-nitrobenzoate, nitrobenzene, 3-dinitrobenzene, 2,6-dinitrotoluene, 3-hydroxy-4-nitrobenzoate, 2,4,6-trinitrophenol, and 4-chlorophenol
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additional information
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almost no activity towards 2,4,6-trinitrophenol
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additional information
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PnpA1A2 also convert 2-chloro-1,4-benzoquinone to 2-hydroxy-4-nitrophenol, the combined isozymes also show low activity with chlorohydroquinone forming 2-hydroxy-1,4-hydroquinone, metabolite identification by GC-MS analysis, overview. Isozyme PnpA2 is not active with 2-chloro-4-nitrophenol
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additional information
?
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Rhodococcus opacus RKJ300
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PnpA1A2 also convert 2-chloro-1,4-benzoquinone to 2-hydroxy-4-nitrophenol, the combined isozymes also show low activity with chlorohydroquinone forming 2-hydroxy-1,4-hydroquinone, metabolite identification by GC-MS analysis, overview. Isozyme PnpA2 is not active with 2-chloro-4-nitrophenol
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additional information
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almost no activity towards 2,4,6-trinitrophenol
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?