1.14.11.45: L-isoleucine 4-hydroxylase
This is an abbreviated version!
For detailed information about L-isoleucine 4-hydroxylase, go to the full flat file.
Word Map on EC 1.14.11.45
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1.14.11.45
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4-hydroxyisoleucine
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corynebacterium
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glutamicum
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thuringiensis
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l-ile
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lactofermentum
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insulinotropic
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stereoselectively
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bl21de3
- 1.14.11.45
-
4-hydroxyisoleucine
-
corynebacterium
- glutamicum
- thuringiensis
- l-ile
- lactofermentum
-
insulinotropic
-
stereoselectively
-
bl21de3
Reaction
Synonyms
IDO, isoleucine 4-hydroxylase, L-isoleucine dioxygenase
ECTree
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Engineering
Engineering on EC 1.14.11.45 - L-isoleucine 4-hydroxylase
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N126H
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mutant obtained by directed evolution, 2.8fold increase in activity
N126H/T130K
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catalytic efficiency is 2.4fold and 3.0fold higher than wild-type with Ile and 2-oxoglutaric acid as substrates
T130K
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mutant obtained by site-specific mutagenesis, 1.7fold increase in activity
I162T/T182N
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double mutant with improvements in specific activity, protein expression level, and fermentation titer of 3.2-, 2.8-, and 9.4fold, respectively
L27I/E80D/G169H/S182D
catalytic efficiency, thermal stability and catalytic rate are significantly improved compared with those of wild-type IDO
additional information
mutants with improved activity are obtained by a strategy dependent on the restoration of succinate-minus Escherichia coli cell growth by the coupling of L-Ile hydroxylation and the oxidation of 2-oxoglutarate to succinate