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1 - 2
-
purified enzyme, 10 min, loss of 90% activity
697664
1.5 - 11
-
enzyme is relatively stable
742681
10.4
-
1 h, 91% loss of activity
765371
2 - 10
-
purified enzyme, 90 min, stable at pH 6.5-9.5, 20% remaining activity at pH 2.0, 65% remaining activity at pH 4.0 and pH 10.0
701029
2 - 11
1 h, stable
742425
2 - 6
-
purified isozymes, 25°C, 1 week, stable
698523
2 - 7
6 h, 25°C, in the absence of the substrate, recombinant laccase is stable
741665
2.5 - 10
recombinant enzyme, rLac1 retains more than 80% of its activity in solutions within this range after a 24 h incubation at 25°C
726411
2.5 - 4
-
purified enzyme, stable
675327
3 - 10
stability of recombinant wild-type and mutant enzymes, overview
697668
3 - 11
Sporothrix carnis
-
2 h, enzyme retains over 60% of its initial activity
742691
3 - 5
-
stable, pH stability profile
699029
3 - 5.6
after treatment with citrate-phosphate buffer (pH 3.0-5.6) for 24 h, enzyme activity is not lost
743888
3 - 6
-
1 h, stable
743016
3 - 6.5
-
stable, purified enzyme
672747
3 - 7
-
purified laccase is stable at 20°C, pH 7.0 and pH 3.0
671510
3.5
24 h, complete loss of activity, recombinant enzyme
742954
3.8
-
1 h, 47% loss of activity
765371
4 - 11
Thermochaetoides thermophila
-
-
396347
4 - 5
-
enzyme loses 50% activity within 2 days
741648
4 - 9
-
the purified laccase is more stable at alkaline pH of 7.0-9.0 after 24 h than at its optimum pH of 4.0
726534
4.2 - 8
good stability
654297
4.4
-
10 h, 16% loss of activity
743889
4.5
half live of mutant laccase in which sixteen original residues are replaced with those found in the phylogenetically inferred ancestral sequence: 63.0 min (at pH 6.0), 6.1 min (pH 10.5)
742942
4.5 - 10
-
the purified enzyme is highly stable over a broad range of pH values ranging from pH 4.5 to pH 10.0, maintaining 75% of its original activity after incubation at 25°C for 72 h, it is highly stable and resistant under alkaline conditions, with pH values ranging from pH 7.0 to pH 10.0
724573
4.5 - 7
-
purified enzyme, 4°C, 24 h, stable
696857
4.6
-
1 h, 23% loss of activity
765371
5 - 7
-
POXA3a and POXA3b, at pH 7.0 after 7 days 50% residual activity
655374
5.4
-
1 h, 17% loss of activity
765371
5.5 - 6.5
-
most stable within pH of 5.5-6.5
764373
5.5 - 8
the L559A mutant remains stable within this pH range after 330 h at 4°C, the enzyme looses 60% at pH 5.0, and 95% at pH 4.0 after 330 h. No activity remains at pH 3.0 and pH 2.0 after 330 h
697913
5.8
-
2 h, 30% loss of activity
741501
6 - 8
-
stable within this range
725302
6.2
-
2 h, 27% loss of activity
741501
6.5 - 9
-
24 h, 37°C, the enzyme retains more than 75% of initial activity
742682
6.6
-
1 h, no loss of activity
765371
6.8
half-life: 6 months
764445
7 - 11
-
the enzyme retains over 50% residual activity after 5-day incubation
741648
8.8
-
1 h, 79% loss of activity
765371
9.4
-
1 h, 86% loss of activity
765371
10
-
purified laccase rapidly loses its activity at pH 10
671510
10
-
1 h, 88% loss of activity
765371
10
-
isozyme Lacc II is stable, isozyme Lacc I loses 10% of activity within 1 h
671514
10
-
24 h, 25°C, about 65% loss of activity
743078
10
half-life are 30 days
697668
10
-
highest stability is observed at pH 10.0 with an approximately residual activity of 90% after 5 days
741648
10
MK290990.1
180 min, 82.84% residual activity
764368
11
24 h, 40% loss of activity
743279
11
Sporothrix carnis
-
2 h, no loss of activity
742691
2
30°C, 40% of enzymatic activities after 1 h treatment
743577
2
mutant laccase 7D5 at pH 2 is dramatically diminished after 24 h
764876
2
PM1 laccase displays high stability at extreme pH values. It retains 60% of the initial activity after 24 h at pH 2
764876
2
activity decreases to approximately 1% at the end of 105 h incubation. t1/2: 18 h
743648
2
-
10 h, residual activity is 3.13%
743889
2
20°C, 2 days, the enzyme retains about 30% residual activity
742013
2.5
-
30°C, 8 min, 50% residual activity
655377
2.5
-
33% loss of activity after 24h
654307
2.5
-
2 h, 22°C, 20% loss of activity
765670
3
-
30°C, 12 min, 50% residual activity
655377
3
-
1 h, 79% loss of activity
765371
3
unstable at, 23 and 15% of the original activity remains after incubation for 1 and 3 days respectively
741634
3
-
10 days, 75-80% loss of activity (depending on purification method)
743713
3
activity decreases to approximately 20% at the end of 105 h incubation. t1/2: 43 h
743648
3
loss of 50% activity after 43 h, 25°C
726424
3
-
partial inactivation of both isozymes, 60-64% remaining activity after 1 h
671514
3
-
25°C, 1 h, stable
688328
3
-
4-25°C, 24 h, loss of 40% activity
697663
3
-
complete loss of activity within 1 day
741648
3
20°C, 2 days, the enzyme retains about 42% residual activity
742013
3 - 9
stable up to when using the substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
764877
3 - 9
-
after 24 h incubation at pH 4.8 and 4°C, 80% of the initial activity remains. After 1 h incubation, the enzyme shows about 65% activity at pH 3.0, about 75% activity at pH 4.0, about 80% activity at pH 5.0, about 60% activity at pH 6.0, about 10% activity at pH 7.0, about 5% activity at pH 8.0, and no activity at pH 9.0
711502
3 - 9
-
purified recombinant enzyme, 48 h, 30°C, completely stable
695796
4
-
18°C, 1 week, 50% residual activity
655377
4
105 h, 56% residual enzyme activity is retained
743648
4
loss of 50% activity after 82 h, 25°C
726424
4
-
24 h, 25°C, about 60% loss of activity
743078
4
-
15% loss of activity after 24h
654307
4
-
2 h, 22°C, no loss of activity
765670
4
-
1 h, most stable at pH 4.0
742638
4
MK290990.1
180 min, 66.45% residual activity
764368
4
20°C, 2 days, the enzyme retains about 65-60% residual activity
742013
4
-
4 h, solgel immobilized laccase retains 98% of the activity. In comparison to soluble laccase better stability is observed in the order, solgel matrix > calcium alginatechitosan > copper alginate > calcium alginate
743017
4 - 10
-
-
396358
4 - 10
-
the laccase has a broad stable pH range. After 72 h incubation at 30°C, the enzyme shows a different stability pattern: the laccase activity decreases rapidly at pH 2.0, 43% remaining activity at pH 4.0 and pH 8.0, over 70% remaining activity at pH 10.0 with a half-life of more than 130 h, the laccase is more stable in an alkaline condition
695714
4 - 6
Myrioconium sp.
-
15°C, 1 h, more than 80% of the initial laccase activity is retained
684626
4 - 6
the enzyme is stable
764898
4 - 8
the laccase is most stable at pH 4.0, pH 7.0, and 8.0, retaining about 57%, 62%, and 65% of the initial activity, respectively, after 355 h. The enzyme is less stable at pH 5.0 and pH 6.0 and is completely inactivated within 355 h at pH 2.0
695839
4 - 8
-
the purified enzyme retains its original activity after 24 h of incubation at pH between 4.0 and 8.0 and after 3 h of incubation at 70°C, pH 7.2 and supplemented with 1.3 M (NH4)2SO4
673378
5
24 h, 25% loss of activity
743279
5
or above, 105 h, more than 80% residual enzyme activity is retained
743648
5
loss of 20% activity after 100 h, 25°C
726424
5
-
2 h, 22°C, 5% loss of activity
765670
5
-
25°C, 1 h, about 15% loss of activity
688328
5
20°C, 2 days, the enzyme retains about 65-60% residual activity
742013
5
-
the enzyme is most stable at pH 5.0 when exposed for 1 h
743786
5
-
moderately stable
685639
5
-
the enzyme iss the most stable at pH 5.0 after 1 h reaction
741664
5 - 10
-
stable at pH 5.0-10.0. Activity is drastically reduced at pH values lower than 5.0 after 6 h incubation
742104
5 - 10
Thermochaetoides thermophila
-
1 h, stable
737487
5 - 6
good stability in the pH range 5.0-6.0
763988
5 - 6
-
4 h, solgel immobilized laccase retains 100% of the activity. In comparison to soluble laccase better stability is observed in the order, solgel matrix > calcium alginatechitosan > copper alginate > calcium alginate
743017
5.5
-
25°C, 1 h, about 55% loss of activity
688328
5.5
24 h, 40% of activity
764445
5.5
-
120 min, the enzyme retains about 82% activity
764373
6
-
1 h, 1% loss of activity
765371
6
-
less than 3% loss of activity after 24h
654307
6
-
30°C, stable for 16 h
656729
6
-
25°C, 1 h, about 80% loss of activity
688328
6
-
120 min, the enzyme retains about 100% activity
764373
6 - 10
no loss of activity after 100 h, 25°C
726424
6 - 10
20°C, 2 days, the enzyme retains about 70% residual activity
742013
6 - 7
25°C, no decrease of activity for at least 5 days
743711
6 - 7
-
purified enzyme, the enzyme retains 80% of its initial activity after 96 h
726416
6 - 7
-
very stable at pH between 6 and 7
685639
6 - 9
-
24 h, 25°C, stable
743078
6 - 9
-
the enzyme retains more than 80% of its activity at within 24 h at 37°C
743704
6 - 9
Phlebia fascicularia
-
90% residual activity after 24 h incungation
657149
6 - 9
-
1 h, stable
718608
6.5
72 h, 22°C, enzyme retains 21% of its activity, recombinant enzyme
742954
6.5
wild-type enzyme Lcc9 and recombinant enzyme expressed in Pichia pastoris retain about 60% of their original activities
765709
6.5
-
2 h, 18% loss of activity
741501
6.5
-
120 min, the enzyme retains about 93% activity
764373
7
-
1 h, 2% loss of activity
765371
7
37°C, no loss of laccase activity after 10 days of incubation
741634
7
-
10 days, 30-40% loss of activity (depending on purification method)
743713
7
-
isozyme Lacc I and isozyme Lacc II, stable
671514
7
-
purified enzyme, 10 min, loss of 90% activity
697664
7
-
4-25°C, 24 h, stable
697663
7
MK290990.1
180 min, 102.02% residual activity
764368
7
-
4 h, solgel immobilized laccase retains 96% of the activity. In comparison to soluble laccase better stability is observed in the order, solgel matrix > calcium alginatechitosan > copper alginate > calcium alginate
743017
7.6
half live of mutant laccase in which sixteen original residues are replaced with those found in the phylogenetically inferred ancestral sequence: 57.8 min (at pH 6.0)
742942
7.6
-
1 h, 10% loss of activity
765371
8
-
1 h, enzyme retains 100% of its activity
743619
8
-
1 h, 48% loss of activity
765371
8
-
10 days, 10-20% loss of activity (depending on purification method)
743713
8
-
more stable at alkaline values of pH (8.0) than at acidic values
687338
8
-
4°C, 24 h, loss of 25% activity, at 25°C oss of 55% activity
697663
8
MK290990.1
180 min, 119.53% residual activity
764368
8
24 h, residual activity of laccase 3 is 89%, residual activity of deglycosylated laccase 3 is 77%
742679
8
-
moderately stable
685639
8
-
4 h, solgel immobilized laccase retains 92% of the activity. In comparison to soluble laccase better stability is observed in the order, solgel matrix > calcium alginatechitosan > copper alginate > calcium alginate
743017
8.5
72 h, 22°C, enzyme retains 17% of its activity, recombinant enzyme
742954
8.5
1 h, 60°C, wild-type enzyme Lcc9 retains 11.8% of its original activity while the recombinant enzyme expressed in Pichia pastoris retains 63.2% of its original activity
765709
9
30°C, 5.5% of enzymatic activities after 1 h treatment
743577
9
24 h, 15% loss of activity
743279
9
half-life: 15 days
764445
9
37°C, no loss of laccase activity after 10 days of incubation
741634
9
-
24 h, the enzyme retains 75% of its activity
743661
9
mutant laccase 7D5 retains 75% activity after 24 h at pH 9
764876
9
PM1 laccase displays high stability at extreme pH values. It retains 100% of the initial activity after 24 h at pH 9
764876
9
MK290990.1
180 min, 86.45% residual activity
764368
additional information
-
stable at alkaline pH
764445
additional information
stable at alkaline pH
764445
additional information
-
unstable at alkaline pH
396341
additional information
-
unstable at alkaline pH
396341
additional information
-
-
671391