   2.3.1.B46 | more |
construction of mboa-7 mutants, the following mutations are used: fat-1(wa9), fat-3(wa22), fat-4(wa14), eri-1(mg366), and mboa-7(gk399). mboa-7(gk399) mutation is backcrossed at five times, phenotype, detailed overview. fat-4 fat-1 mutants, which do not produce AA(20:4n-6) and n-3 PUFAs such as EPA(20:5n-3), are viable and fertile, but they exhibited slow growth at 15°C. Dietary supplementation with arachidonic acid (AA) or eicosapentaenoic acid (EPA) leads to incorporation of these PUFAs into the phospholipid fractions of fat-4 fat-1 mutants, and it rescues the growth defects of this strain. Fatty acid composition of wild-type and mboa-7 mutants, overview |
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