EC Number   |
Protein Variants |
Reference |
|---|
   2.3.1.48 | A190S |
site-directed mutagenesis, mutant of Gcn5, similar kinetics to wild-type |
487102 |
| 2.3.1.48 | A190T |
site-directed mutagenesis, mutant of Gcn5, decreased Km for acetyl-CoA |
487102 |
| 2.3.1.48 | C303A |
site-directed mutagenesis of Sas3, exchange in zinc finger motif, remaining activity: 4.5% of wild-type activity |
487091 |
| 2.3.1.48 | C304A |
site-directed mutagenesis, the mutant shows activity and pH-dependence similar to the wild-type enzyme |
672263 |
| 2.3.1.48 | C304S |
site-directed mutagenesis, the mutant shows 97.4% reduced histone H4 acetylation at pH 8.0 compared to the wild-type Esa1 |
703867 |
| 2.3.1.48 | C304S |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme |
672263 |
| 2.3.1.48 | C304S/E338Q |
site-directed mutagenesis, the mutant protein is intrinsically unstable and catalytically inactive, the mutant cell is lethal |
703867 |
| 2.3.1.48 | C306A |
site-directed mutagenesis of Sas3, exchange in zinc finger motif, remaining activity: 11.1% of wild-type activity |
487091 |
| 2.3.1.48 | C323A |
site-directed mutagenesis of Sas3, exchange in zinc finger motif, no remaining activity |
487091 |
| 2.3.1.48 | C732W |
mutation abolishes the binding to histone H3 |
721058 |
