EC Number |
Protein Variants |
Reference |
---|
2.1.1.148 | E190G |
does not confer thymidine-independent growth to an Escherichia coli strain lacking thymidylate synthase ThyA, is capable of binding FAD at a wild-type level, but lacks detectable oxidation and deprotonation |
680675 |
2.1.1.148 | E190G |
no detectable activity |
659429 |
2.1.1.148 | H177K |
does not confer thymidine-independent growth to an Escherichia coli strain lacking thymidylate synthase ThyA, 9.5% oxidation activity |
680675 |
2.1.1.148 | H177Q |
confers thymidine-independent growth to an Escherichia coli strain lacking thymidylate synthase ThyA, 31% oxidation activity |
680675 |
2.1.1.148 | H48Q |
inactive mutant enzyme |
660374 |
2.1.1.148 | H48Q/S84A |
inactive mutant enzyme |
660374 |
2.1.1.148 | H48Q/S84C |
inactive mutant enzyme |
660374 |
2.1.1.148 | H53D |
residue H53 is involved in folate binding. Crystal structures of the H53D-FAD and H53D-FAD-dUMP complexes |
-, 734346 |
2.1.1.148 | H53K |
does not confer thymidine-independent growth to an Escherichia coli strain lacking thymidylate synthase ThyA, produces insoluble protein |
680675 |
2.1.1.148 | H53Q |
does not confer thymidine-independent growth to an Escherichia coli strain lacking thymidylate synthase ThyA, produces insoluble protein |
680675 |