EC Number   |
Protein Variants |
Reference |
|---|
    1.8.1.7 | A18E//N21W/R22N/N101D/K105D/R319A |
mutant enzyme shows very little activity with GSSG in micromolar range but, on increasing the GSSG concentration to 20 mM some activity with GSSG can be detected. 1.5fold decrease in turnover-number for trypanothione, 9.5fold decrease in KM-value for trypanothione |
663260 |
| 1.8.1.7 | A18E/N21W/R22N |
mutant enzyme shows very little activity with GSSG in micromolar range but, on increasing the GSSG concentration to 20 mM some activity with GSSG can be detected. 1.6fold increase in turnover-number for trypanothione, 3fold decrease in KM-value for trypanothione |
663260 |
| 1.8.1.7 | A18E/N21W/R22N/N101D/K105D |
mutant enzyme shows very little activity with GSSG in micromolar range but, on increasing the GSSG concentration to 20 mM some activity with GSSG can be detected. 1.5fold increase in turnover-number for trypanothione, 6.7fold decrease in KM-value for trypanothione |
663260 |
| 1.8.1.7 | A34E/R37W |
glutathione reductase mutant, activity switches to trypanothione reductase, termed GRTR, 700fold more activity with trypanothione than with glutathione |
394776 |
| 1.8.1.7 | more |
a mutant lacking a loop involved in ligand binding, showing reduced activity, and a mutant with a modified loop, which is more efficient with NADPH and NADH |
394772 |
| 1.8.1.7 | more |
analysis of enzyme import rates into chloroplasts of diverse point mutated pea strains, overview |
660197 |
| 1.8.1.7 | more |
cell-free enzyme synthesis, 71% of which is soluble, the enzyme is dimeric, requires FAD as a cofactor, and is more active than the native enzyme |
658730 |
| 1.8.1.7 | more |
construction of a deletion mutant lacking residues 317-349 forms a dimeric FAD-free apoenzyme |
659698 |
| 1.8.1.7 | more |
introduction of silent random mutations in 5'-region to generate a His-tagged high-level expression clone |
394767 |
| 1.8.1.7 | more |
N278 at the vicinity of NADP-binding pocket is artificially glycosylated when heterogeneously overexpressed in Pichia pastoris. The highly motile oligosaccharide chain linked to N278 of the recombinant Glr1 interferes with the entry of NADPH, wich results in a dramatic increase of Km for NAPDH and a significant decrease of turnover number, when compared with the native protein |
689967 |
