EC Number   |
Protein Variants |
Reference |
|---|
    1.14.15.30 | D230E/S232T/F238Y |
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme |
-, 717737 |
| 1.14.15.30 | D230E/S232T/F238Y |
the mutant of isoform KshA1 shows no 3-ketosteroid 9alpha-hydroxylase activity |
717737 |
| 1.14.15.30 | D242W |
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme |
717737 |
| 1.14.15.30 | D242W |
the mutant of isoform KshA1 exhibits 3-ketosteroid 9alpha-hydroxylase activity |
717737 |
| 1.14.15.30 | E236D/T238S/Y244F |
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme |
717737 |
| 1.14.15.30 | E236D/T238S/Y244F |
the mutant of isoform KshA5 shows no 3-ketosteroid 9alpha-hydroxylase activity |
717737 |
| 1.14.15.30 | more |
a 5fold kshA null mutant of strain DSM43269 (strain RG32) lacks genes kshA1, kshA2, kshA3, kshA4, and kshA5. Contrary to wild type DSM43269, the kshA null mutant strain RG32 shows no growth on progesterone, 4-androstene-3,17-dione, or cholic acid. Growth of strain RG32 on cholesterol is severely impaired, but not completely blocked |
-, 717735 |
| 1.14.15.30 | more |
a 5fold kshA null mutant of strain DSM43269 (strain RG32) lacks genes kshA1, kshA2, kshA3, kshA4, and kshA5. Contrary to wild type DSM43269, the kshA null mutant strain RG32 shows no growth on progesterone, 4-androstene-3,17-dione, or cholic acid. Growth of strain RG32 on cholesterol was severely impaired, but not completely blocked |
-, 717735 |
| 1.14.15.30 | more |
a kshA null mutant is constructed by gene deletion mutagenesis (strain RG32) to fully block opening of the steroids polycyclic ring structure of cholesterol and beta-sitosterol resulting in accumulation of 1,4-androstadiene-3,17-dione and 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid |
-, 744058 |
| 1.14.15.30 | more |
construction of chimeric KshA enzyme mutants with exchanged helix-grip fold beta-sheets that retain KSH activity. kshA1 E198 to V255 is exchanged for kshA5 E204 to I261. kshA1 I257 to I306 is exchanged for kshA5 I263 to V312, kshA5 E204 to I261 is exchanged for kshA1 E198 to V255, kshA5 I263 to V312 is exchanged for kshA1 I257 to I306, kshA1 209-QAREDTRPHANGQPKMIGS-227is exchanged for kshA5 215-TGREDVISGTNYDDPNAEL-233, and kshA5 215-TGREDVISGTNYDDPNAEL-233 is exchanged for kshA1 209-QAREDTRPHANGQPKMIGS-227 |
-, 717737 |
