EC Number   |
Protein Variants |
Reference |
|---|
  1.14.13.101 | F307Y |
site-directed mutagenesis, the ZvFMOa F307Y mutation results in a decline in specific activity by a factor of 0.81 in comparison to wild-type ZvFMOa |
763839 |
| 1.14.13.101 | F307Y/Y356A |
site-directed mutagenesis, the ZvFMOa F307Y/Y356A double mutation results in an increase in specific activity by a factor of 1.51 in comparison to wild-type ZvFMOa |
763839 |
| 1.14.13.101 | F354V |
site-directed mutagenesis, the ZvFMOa F354V mutation results in an increase in specific activity by a factor of 2.75 in comparison to wild-type ZvFMOa |
763839 |
| 1.14.13.101 | F354V/Y356A |
site-directed mutagenesis, the ZvFMOa F354V/Y356A double mutation results in an increase in specific activity by a factor of 1.5 in comparison to wild-type ZvFMOa |
763839 |
| 1.14.13.101 | more |
the beneficial effect of a more accessible substrate entry path might possibly be partly antagonized by a loss of substrate affinity when both bulky aromatic amino acids are exchanged for smaller substituents. The presence of proline within the ZvFMOa helix does not interfere with its potential role in substrate turnover |
763839 |
| 1.14.13.101 | P388S |
site-directed mutagenesis, the specific activity of this variant does not differ significantly from that of wild-type ZvFMOa |
763839 |
| 1.14.13.101 | Y356A |
site-directed mutagenesis, the ZvFMOa Y356A mutation results in an increase in specific activity by a factor of 2.45 in comparison to wild-type ZvFMOa |
763839 |
