  2.4.1.B71 | purified recombinant His-tagged enzyme, sitting-drop vapour-diffusion method, mixing of 0.001 ml of 0.7 mg/ml protein in 20 mM TrisHCl, pH 8.0, 150 mM NaCl, 1 mM dithiothreitol, with 0.001 ml of reservoir solution containing 0.1 M MESNaOH, pH 6.3, 14% w/v PEG 4000, 1 M NaCl, 0.2 M MgCl2, 5mM UDP-glucose, and equilibration against 0.06 ml reservoir solution, 2 days, 20°C, soaking of crystals in 0.1 M MES-NaOH, pH 6.3, 17% w/v PEG 4000, 1 M NaCl, 0.2 M MgCl2, 20mM UDP-glucose, 2 mM quercetin, 20% ethylene glycol, X-ray diffraction structure determination and analysis at 2.2 A resolution |
