2.3.3.20 | hanging drop vapor diffusion method, using 16-21% (w/v) PEG 8000, 70-100 mM potassium phosphate dibasic, and 100 mM sodium citrate pH 4.2 (enzyme only) or 13-18% (w/v) PEG 4000, 80-100 mM manganese chloride, and 100 mM MES 6.0 (enzyme in complex with cerulenin) |