EC Number   |
|---|
   2.3.2.32 | hanging drop vapor diffusion method, using 2% (w/v) PEG3350, 0.1M HEPES, 0.2 M L-Pro, pH 8.0 |
| 2.3.2.32 | hanging drop vapor diffusion method, using 400 mM ammonium acetate, 30% (w/v) PEG4000, and 0.1 M trisodium citrate (pH 5.6) at 20°C |
| 2.3.2.32 | hanging drop vapor diffusion method, using about 20% (w/v) PEG3350, 0.2 M ammonium citrate, pH 7.0 |
| 2.3.2.32 | structures of the neddylated and deneddylated COP9 signalosome-cullin-2 complexes. The structures suggest a conserved mechanism of COP9 signalosome activation, consisting of conformational clamping of the cullin-2 substrate by COP9 signalosome subunits CSN2/CSN4, release of the catalytic CSN5/CSN6 heterodimer and finally activation of the CSN5 deneddylation machinery. Cullin-2 activates CSN5/CSN6 in a neddylation-independent manner |
