1.14.15.4 | purified recombinant N-terminally MAKKTSS-tagged and C-terminally His6-tagged CYP11B2 without mitochondrial targeting peptide complexed with substrate or inhibitor, hanging drop vapor diffusion method, 14 mg/ml protein in 50 mM potassium phosphate, pH 7.4, containing 500 mM NaCl, 0.5% CHAPS, 20% glycerol, and 0.05 mM 11-deoxycortisosterone or 0.05 mM fadrozole, mixing with reservoir solution containing 10% PEG 4000, 0.2 M lithium sulfate, and 0.1 M Tris, pH 8.5, X-ray diffraction structure determination and analysis at 2.5-2.7 A resolution |