EC Number |
---|
1.14.13.22 | - |
1.14.13.22 | enzyme in complex with FAD and NADP+ in 2 distinct states, hanging drop or sitting drop vapor diffusion methods, crystal form I with 0.2 M sodium acetate trihydrate, 0.1 M sodium cacodylate pH 6.5, and 30% PEG 8000, for crystal form II with 0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate pH 6.5, and 20% PEG 8000 |
1.14.13.22 | microdialysis method, saturated ammonium sulfate solution |
1.14.13.22 | sitting drop vapor diffusion method. Two crystal structures of cyclohexanone monooxygenase with its product, epsilon-caprolactone, bound: the CHMO(Tight) and CHMO(Loose) structures. The CHMO(Tight) structure represents the enzyme state in which substrate acceptance and stereospecificity is determined, providing a foundation for engineering BVMOs with altered substrate spectra and/or stereospecificity. The CHMO(Loose) structure is the first structure where the product is solvent accessible. This structure represents the enzyme state upon binding and release of the substrate and product |
1.14.13.22 | structure of variant F246Y/K326C/L426F/F432L/T433A/L435S/S438I/F505L complexed with FAD, to 2.2 A resolution |