EC Number |
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1.1.1.261 | sitting drop vapor diffusion method, using 30% (w/v) poly(ethylene glycol) methyl ether 550/polyethylene glycol 20000 and 0.1 M HEPES, pH 7.6 |
1.1.1.261 | structure with bound substrate dihydroxyacetone phosphate, product glycerol 1-phosphate, NADPH, and Zn2+ cofactor. The sole role of His226, His247, and Asp148 is to coordinate the active site Zn2+. A pro-R hydride transfer/relay system is in operation during catalysis, mediated by water and completed by the polarization of the carbonyl of dihydroxyacetone phosphate by Zn2+. Residue Asn104 may influence affinity for the coenzyme and the rate of reaction via its interaction with NADPH |
1.1.1.261 | to 2.45 A resolution, one homohexamer per asymmetric unit, with presence of the bound cofactor NADPH in subunits D, E, and F. The phosphate group at C2' of the adenine ribose of NADPH is tightly held through the five biased hydrogen bonds with Ser40 and Thr42. The NADPH molecule is pushed away from the normal coenzyme binding site |