EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    2.4.1.13 | 30 |
- |
pH 7.6, 200 mM HEPES-NaOH buffer, 48 h, 20% loss of activity |
659541 |
| 2.4.1.13 | 37 |
- |
t1/2: 5 h, pH 6.5 |
488537 |
| 2.4.1.13 | 37 |
50 |
the enzyme is thermostable up to 50°C at pH 6.5 |
757316 |
| 2.4.1.13 | 45 |
- |
the soluble enzyme shows a half-life of 0.75 h at 45°C. The enzyme immobilized on agarose support coated with polyethyleneimine shows a half-life of 362 h at 45°C |
756962 |
| 2.4.1.13 | 50 |
- |
the enzyme remains stable after 10 min incubation up to 50°C, but activity sharply decays beyond 55°C. Addition of 1 mM ADP, 10 mM UDP, or 200 mM Suc enhances the thermal stability by almost 5°C |
736101 |
| 2.4.1.13 | 50 |
- |
thermostability of wild type isoforms SUS1 and SUS2 is poor at 50°C. Activity loss occurs rapidly with increasing incubation time, and the half-lives are 50 and 25 min for SUS1 and SUS2, respectively. Mutant enzyme SUS1-DELTA9 shows a half-life increase of 2fold. The residual activity is 50% after 100 min, while the wild type activity remains less than 20% of the original activity. The half-life of the variant SUS2-DELTA9 also increases 2fold |
757047 |
| 2.4.1.13 | 55 |
- |
pH 6.5: t1/2: 1 min, complete inactivation after 10 min, pH 8: 20% loss of activity after 1 min, 80% loss of activity within 10 min |
488562 |
| 2.4.1.13 | 60 |
- |
and above, 5 min, rapid loss of activity |
488554 |
| 2.4.1.13 | 60 |
- |
purified recombinant His-tagged enzyme, without substrates, 15 min, 38% activity remaining |
735556 |
| 2.4.1.13 | 60 |
- |
purified recombinant His-tagged enzyme, without substrates, 15 min, 54% activity remaining |
735556 |
