EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
2.3.2.2 | -999 |
- |
melting temperature of enzyme GGT1 is 53-58°C depending on the buffer system and pH used, while with bound inhibitor 6-diazo-5-oxo-L-norleucine, the mellting temperature is 69-72°C, overview. The unbound enzyme is most stable in Na-citrate, pH 6.7, the inhibitor-bound enzyme in HEPES, pH 7.0 |
758292 |
2.3.2.2 | 4 |
30 |
at least 10 min stable |
487950 |
2.3.2.2 | 20 |
70 |
purified native enzyme, the enzyme retains more than 88% of its activity at 20-70°C, but the enzyme activity is significantly decreased at temperatures above 70°C |
757351 |
2.3.2.2 | 37 |
77 |
in both the transpeptidation and hydrolysis reactions, wild type enzyme exhibits full activity up to 47°C. At temperatures greater than 62°C, the wild type enzyme is rendered completely inactive |
719952 |
2.3.2.2 | 40 |
- |
10% loss of activity within 10 min |
487946 |
2.3.2.2 | 40 |
- |
30 min, pH 8.0, stable below |
671541 |
2.3.2.2 | 40 |
- |
at least 15 min stable |
487943 |
2.3.2.2 | 40 |
- |
stable below |
487947 |
2.3.2.2 | 40 |
50 |
there is hardly any activity loss of the purified recombinant enzyme after preincubation at 40 °C for 72 h, but the activity drops to 7.5% after preincubation for 8 h at 50°C |
757049 |
2.3.2.2 | 40 |
60 |
thermal stability of BaGGT469 is extremely low and its residual activity is completely lost within 10 min at 60°C. But BaGGT469 is quite stable in the presence of substrate at 40°C |
757413 |