EC Number   |
|---|
   2.4.1.16 | - |
| 2.4.1.16 | chitin synthase 2 |
| 2.4.1.16 | cloning and expression of class I CHS isozyme |
| 2.4.1.16 | cloning and expression of class III CHS isozyme |
| 2.4.1.16 | cloning and expression of class IV and V CHS isozymes |
| 2.4.1.16 | cloning from anterior midguts of fifth instar larvae, expression in Saccharomyces cerevisiae AH109 cells and protein interaction analysis by yeast two hybrid screening, identifying a chymotrypsin-like protease CTLP1 that binds to the extracellular carboxyterminal domain of CHS2 |
| 2.4.1.16 | comparative sequence analysis, cloning and expression of the enzyme's transmembrane segment ArCS1_E22TM, recombinant expression of the His-tagged domain in Dictyostelium discoideum, the corresponding transmembrane protein ArCS1_E22TM accumulates in membrane fractions of the expression host Dictyostelium discoideum, recombinant expression of clone EIIIab containing the ArCS1_E22 cDNA fragment in Escherichia coli strain BL21(DE3) |
| 2.4.1.16 | expressed in Escherichia coli BL21(DE3) cells |
| 2.4.1.16 | expressed in Saccharomyces cerevisiae using the GAL1 promoter in a multicopy plasmid |
| 2.4.1.16 | expression in CF203/2.5 insect cell |
