EC Number   |
|---|
    2.3.3.16 | - |
| 2.3.3.16 | alfalfa is engineered by introducing the citrate synthase gene controlled by the Arabidopsis Act2 constitutive promoter or the tobacco RB7 root-specific promoter. Fifteen transgenic plants are assayed for exclusion of aluminum from the root tip, for internal citrate content, for growth in in vitro assays, or for shoot and root growth in either hydroponics or in soil assays. Based on the soil assays, two transgenic events are identified that are more aluminum-tolerant than the non-transgenic control, confirming that citrate synthase overexpression can be a useful tool to help achieve aluminum tolerance |
| 2.3.3.16 | cloning and expression in Aspergillus nidulans, DNA sequence |
| 2.3.3.16 | cloning and expression of citA in Aspergillus niger, 11fold overexpression does not increase the citrate activity level in vivo |
| 2.3.3.16 | cloning and expression of wild-type and mutants in Escherichia coli |
| 2.3.3.16 | cloning and sequencing |
| 2.3.3.16 | cloning of His-tagged fusion protein of citrate synthase and mitochondrial malate dehydrogenase, expression in Escherichia coli |
| 2.3.3.16 | codon-optimized expression in Escherichia coli |
| 2.3.3.16 | ectopic overexpression in Arabidopsis thaliana strain WS via infection with Agrobacterium tumefaciens, DNA sequence determination |
| 2.3.3.16 | expressed in Arabidopsis thaliana |
