EC Number   |
|---|
    2.3.2.2 | - |
| 2.3.2.2 | a truncated Escherichia coli Novablue gamma-glutamyltranspeptidase gene lacking the first 48-bp coding sequence for part of the signal sequence is amplified by polymerase chain reaction and cloned into expression vector pQE-30 to generate pQE-EcGGT. The maximum production of His6-tagged enzyme by Escherichia coli M15 is achieved with 0.1 mM IPTG induction for 12 h at 20°C |
| 2.3.2.2 | cloning of liver specific isoform III from fetal mRNA, screening of rat genetic library, single copy gene, DNA, including enhancer and promotor, sequence determination, plasmid construction and expression in Escherichia coli, transient enzyme expression in hepatoma cell lines, dependent on differentiation state of the cells |
| 2.3.2.2 | expressed in Escherichia coli BL21 cells |
| 2.3.2.2 | expressed in Escherichia coli BL21(DE3) cells |
| 2.3.2.2 | expressed in Escherichia coli C41(DE3) cells |
| 2.3.2.2 | expressed in Escherichia coli M15 cells |
| 2.3.2.2 | expressed in Pichia pastoris |
| 2.3.2.2 | expressed in Pichia pastoris and in HEK-293 cells |
| 2.3.2.2 | expression in CHO cells |
