EC Number   |
|---|
   2.3.1.26 | - |
| 2.3.1.26 | a recombinant baculovirus transfer vector is constructed by subcloning of human ACAT-1 cDNA into pFASTBacHis |
| 2.3.1.26 | a recombinant baculovirus transfer vector is constructed by subcloning of human ACAT-2 cDNA into pFASTBacHis |
| 2.3.1.26 | a stable cell line expressing hACAT-1 from AC-29 cells is established |
| 2.3.1.26 | a stable cell line expressing hACAT-2 from AC-29 cells is established |
| 2.3.1.26 | all constructs, full-length, chimeras and mutants are cloned into the pCDNA3 vector, AC29 cells are transfected |
| 2.3.1.26 | cDNA cloned |
| 2.3.1.26 | CHO cells stably overexpressing human ACAT-1 are used |
| 2.3.1.26 | CHO cells stably overexpressing human ACAT-2 are used |
| 2.3.1.26 | expressed as a His-tagged fusion protein in Escherichia coli |
