EC Number   |
|---|
   2.1.1.246 | gene mtaA, DNA and amino acid sequence determination and analysis, gene mtaA is monocislronically transcribed, transcription of the gene is positively controlled by the growth substrate rnethanol, overexpression of the His6-tagged enzyme in Escherichia coli strain M15 |
| 2.1.1.246 | gene mtaA, expression of His-tagged MtaA in Escherichia coli strain M15 |
| 2.1.1.246 | gene mtaA, recombinant expression as enzyme complex Methanosarcina barkeri MtaABC in Escherichia coli, recombinant expression of His-tagged MtaA in Escherichia coli |
| 2.1.1.246 | gene mtaA, sequence comparison, expression of His-tagged MtaA in Escherichia coli strain M15 |
| 2.1.1.246 | genes cmtA and cmtM, DNA and amino acid sequence determination and analysis, expressionin Escherichia coli strain XL-1 Blue |
| 2.1.1.246 | genes mtaA and mtaB, expression of N-terminally His-tagged isozymes MtaA and MtbA in Escherichia coli strain M15 |
| 2.1.1.246 | genes mtaA1 and mtaA2, overexpression of N-terminally His6-tagged mtaA1 in Escherichia coli strain BL21(DE3), strains carrying various combinations of mtaC, mtaB, and mtaA expressed from the strong, tetracycline-regulated PmcrB(tetO1) promoter exhibit similar growth characteristics on methanol, showing that all combinations of MtaC, MtaB, and MtaA can form functional MT1/MT2 complexes. Variations in activity correlate with differences in protein abundance, despite the fact that all the encoding genes are expressed from the same promoter, overview. The mtaCBA transcripts show different stabilities, which are strongly influenced by the growth substrate, quantitative expression and activity analysis, overview |
| 2.1.1.246 | overexpression of His-tagged inactive MtaA apoprotein in Escherichia coli strain M15 grown in the presence of 2 mM EDTA |
