EC Number   |
|---|
   1.3.99.5 | expression in Escherichia coli JM109 carrying pXE25, activity is maximal 18times higher than the activity expressed by induced cells of Comamonas testosteroni. Overexpression in Pseudomonas putida KT2440 using pMMB208 as expression vector, activity is maximaly 264times higher than the activity expressed by induced cells of Comamonas testosteroni |
| 1.3.99.5 | gene SRD5A2, DNA and amino acid sequence analysis in 6 Korean patients with 5alpha-reductase type 2 deficiency, overview |
| 1.3.99.5 | gene SRD5A2, genotyping of 114 prostate cancer patients and 144 healthy control males for single-nucleotide polymorphisms, A49T and V89L, overview. Individuals with LL genotype show reduction in the progression to a higher tumor stage |
| 1.3.99.5 | phylogenetic analysis of SRD5alpha, overview. SRD5alpha subfamilies include, not only the well-known SRD5alpha type 1, type 2 and type 3, but also the synaptic glycoprotein (GPSN2)/trans-2,3-enoly-CoA reductase group |
| 1.3.99.5 | phylogenetic analysis of SRD5alpha, overview. SRD5alpha subfamilies include, not only the well-known SRD5alpha type 1, type 2 and type 3, but also the synaptic glycoprotein (GPSN2)/trans-2,3-enoly-CoA reductase group. SRD5alpha1 is located on chromosome 5p15 whereas SRD5alpha2 is located on 2p22, and SRD5alpha3 is located at 4q12 |
| 1.3.99.5 | quantitative expression analysis of isozymes 5alpha-R1 and 5alpha-R2 in brain |
| 1.3.99.5 | Srd5a1 DNA and amino acid sequence determination and analysis, expression of Srd5a2 in HEK-293 cells, real-time quantitative RT-PCR expression analysis with higher mRNA levels for Srd5a1 than for Srd5a2 |
| 1.3.99.5 | Srd5a2 DNA and amino acid sequence determination and analysis, expression of Srd5a2 in HEK-293 cells, real-time quantitative RT-PCR expression analysis with higher mRNA levels for Srd5a1 than for Srd5a2 |
| 1.3.99.5 | sterol regulatory element binding proteins, SREBPs, regulate expression of Srd5a2. 293T cells are transfected with the Srd5a2 promoter-luciferase reporter and flag-pCDNA3.1+ SREBP-2 plasmids. SREBP-2 binds to the promoter for Srd5a2 |
| 1.3.99.5 | transient expression of pTRE-5alphaR1 in LNGK-9 PCa cells. Addition of testosterone increases growth of pTRE-5alphaR1 transfectants by 54.1% over cells grown in the absence of testosterone, compared to 25.0% in control cells. Testosterone also increases secretion of prostate-specific antigen 17fold in the 5alphaR1-transfected cells |
