EC Number |
---|
1.3.1.33 | - |
1.3.1.33 | a genomic library is constructed, the vector pZERO is used |
1.3.1.33 | barley POR expressed in Escherichia coli as a beta-galactosidase fusion protein |
1.3.1.33 | cDNA library constructed in pBR322, full-length cDNA cloned, cDNA inserts subcloned in the M13 phage and expressed in Escherichia coli |
1.3.1.33 | cDNAs and/or gens encoding POR, also referred to as LPCR or PCR in some studies isolated |
1.3.1.33 | cloned and overproduced in Escherichia coli with a hexahistidine tag at the N-terminus |
1.3.1.33 | cloning of the gene encoding protochlorophyllide reductase |
1.3.1.33 | cloning of the nuclear gene |
1.3.1.33 | expressed in Escherichia coli |
1.3.1.33 | expressed in Escherichia coli BL21(DE3) cells |