EC Number   |
|---|
    1.23.5.1 | a full-length violaxanthin de-epoxidase and deletion mutants of the N- and C-terminal regions are expressed in Escherichia coli and Nicotiana tabacum L. cv. Xanthi. High expression of the enzyme in Escherichia coli is achieved after adding the argU gene that encodes the Escherichia coli arginine AGA tRNA. The specific activity of the violaxanthin de-epoxidase expressed in Escherichia coli is low, possibly due to incorrect folding. The transformed tobacco exhibits a 13fold to 19fold increase in VDE specific activity, indicating correct protein folding |
| 1.23.5.1 | DNA and amino acid sequence determination and analysis, phylogenetic analysis, transiently expression of GFP-tagged enzyme in cucumber protoplasts, anti-sense expression of CsVDE in Arabidopsis thaliana leading to silencing of the single copy Arabidopsis thaliana gene, quantitative real-time PCR expression analysis |
| 1.23.5.1 | expressed in Escherichia coli Origami B cells |
| 1.23.5.1 | expressed in Lycopersicom esculentum |
| 1.23.5.1 | expressed in Nicotiana tabacum |
| 1.23.5.1 | expressed in Nicotiana tabacum NC89 |
| 1.23.5.1 | expression in Escherichia coli |
| 1.23.5.1 | gene AhVDE, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Nicotiana tabacum, coexpression with anti-sense tobacco RNAi-NtVDE, semiquantitative and quantitative RT-PCR expression analysis |
| 1.23.5.1 | gene VDE1, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) in inclusion bodies |
| 1.23.5.1 | gene VDE1, recombinant expression of wild-type and truncated mutants in Escherichia coli strain BL21(DE3) in inclusion bodies |
