1.17.99.10 | a heterologous expression platform is established for the three structural genes of S25DH subunits together with an essential chaperone in the denitrifying betaproteobacterium Thauera aromatica K172. Using this system, steroid C-25 dehydrogenase S25DH1 and three isoenzymes (S25DH2, S25DH3, and S25DH4) are overproduced in a soluble, active form allowing a straightforward purification of nontagged alphabetagamma complexes |