EC Number   |
|---|
   1.14.11.65 | expressed in Mus musculus |
| 1.14.11.65 | expression in HEK293T cell |
| 1.14.11.65 | expression of FLAG-tagged wild-type and H190A mutant Jmjd2c in mouse embryonic fibroblasts |
| 1.14.11.65 | gene At4g00990, quantitative and nonquantitative reverse transcription PCR enzyme expression analysis, construction of transgenic plants expressing a JMJ27 promoter-GUS fusion, A sequence containing approximately 1850 bp immediately upstream of the JMJ27 translation start site is fused to the beta-glucuronidase (GUS) reporter gene, and the construct is transformed into wild-type plants. GUS analysis is carried out at various stages of plant growth and in different organs. JMJ27 promoter activity is detected in 7-day-old seedlings, flowers and siliques. A reporter construct containing JMJ27 cDNA fused in-frame to GFP cDNA is and expressed from the constitutive 35S promoter (35S::JMJ27-GFP) in onion epidermal cells in the nucleus. Recombinant enzyme expression as JMJ27-MBP fusion protein |
| 1.14.11.65 | gene HR, real-time RT-PCR enzyme expression analysis, ectopic expression of wild-type protein hairless, HR, but not JmjC-mutant HR, leads to pronounced demethylation of H3K9 in cultured human HeLa cells, reombinant expression of Flag-tagged HR in HEK-293 cells |
| 1.14.11.65 | gene jmj25, promoter-GUS fusion analysis and histochemical assay of GUS activity of wild-type and mutants |
| 1.14.11.65 | gene KDM1A, codon optimized, recombinant expression of the enzyme LSD1, residues 151-852, in Escherichia coli |
| 1.14.11.65 | gene KDM1A, real-time PCR enzyme expression analysis |
| 1.14.11.65 | gene KDM1A, recombinant expression of full-length His6-tagged human LSD1 from pET15b bacterial expression vector in Escherichia coli strain BL21(DE3) |
| 1.14.11.65 | gene KDM1A, recombinant expression of N-terminally His-tagged full-length LSD1 protein and deletion mutant DELTA184 LSD1lacking the N-terminal 184 amino acid residues |
