EC Number   |
|---|
   1.10.3.1 | AoCO4 is expressed in Trichoderma reesei under the strong cbh1 promoter, resulting in high yields of extracellular enzyme, corresponding to 1.5 g/l production of the enzyme |
| 1.10.3.1 | cloning of cDNA |
| 1.10.3.1 | expressed in Escherichia coli BL21(DE3) cells |
| 1.10.3.1 | expressed in Escherichia coli BL21(DE3) cells using pET30c expression vector (high expression) and in Pichia pastoris GS115 using both the secretory and non-secretory vectors pPICZ-A and pPICZA (low expression) |
| 1.10.3.1 | expressed in Escherichia coli BL21(deltaDE3) cells |
| 1.10.3.1 | exression in Trichoderma reesei |
| 1.10.3.1 | gene ppo-6, expression of N-terminally Strep2-tagged isozyme PPO-6 in Escherichia coli strain Rosetta2(DE3) pLysSRARE2 |
| 1.10.3.1 | gene RSc0337, sequence comparison |
| 1.10.3.1 | gene tyr2, DNA and amino acid sequence determination and analysis, overexpression in the native host under the strong cbh1 promoter, secretion of the recombinant enzyme into the culture supernatant |
