EC Number   |
Storage Stability |
|---|
   1.1.1.149 | -15°C, 0.005 mg/ml, 0.01 M potassium phosphate buffer, pH 8.0, 50% propylene glycol, 0.2 M KCl, 2 months |
| 1.1.1.149 | -20°C, porcine testes, 0.1 M phosphate buffer, pH 7.00.1 M phosphate buffer, pH 7.0, several months |
| 1.1.1.149 | -20°C, purified enzyme, 10 mM Tris/HCl, pH 7.5, 0.5 mM EDTA, 1 mM 2-mercaptoethanol, 20%, v/v, glycerol, 2 weeks, little activity loss |
| 1.1.1.149 | -70°C, 2.8 mg/ml protein concentration, 20 mM potassium phosphate buffer, pH 7.0, 1 mM EDTA, 1 mM beta-mercaptoethanol, 30%, v/v, glycerol |
| 1.1.1.149 | -80°C, particulate and cytosolic fraction after homogenization |
| 1.1.1.149 | 4 or -20°C, 2 mg/ml purified protein, 0.1 M phosphate buffer, pH 6-9, several days or for months |
| 1.1.1.149 | 4°C or -20°C, above 0.5 mg of purified protein per ml, 50 mM phosphate buffer, pH 6.8, 10 mM 2-mercaptoethanol, 50% glycerol, v/v, several days |
| 1.1.1.149 | 4°C or -20°C, ammonium sulfate fraction of purification step 2, over night or several months |
| 1.1.1.149 | 4°C pH 6-7 |
| 1.1.1.149 | 4°C, cytosol preparation prior to purification, several months |
