1.3.7.15 | robust reconstitution of recombinant COR activity is achieved by coupling it to another enzymatic assay using the green pigment, protochlorophyllide, as a substrate of the nitrogenase-like enzyme, dark-operative protochlorophyllide oxidoreductase (DPOR). The DPOR reaction results in the formation of chlorophyllide (Chlide), which then acts as a direct substrate of COR. Reconstitution of DPOR and COR activities requires high concentrations of ATP, as well as a suitable electron donor |
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