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EC Number	Renatured (Commentary)	Reference
1.23.5.1	recombinant enzyme from Escherichia coli strain BL21(DE3) inclusion bodies by solibilization with 8 M urea, 60 mM Tris-HCl, pH 8.0, 60 mM NaCl, and 0.6 mM EDTA, followed by dialysis and ultracentrifugation	745996
1.23.5.1	recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) inclusion bodies by solibilization with 8 M urea, 60 mM Tris-HCl, pH 8.0, 60 mM NaCl, and 0.6 mM EDTA, followed by dialysis and ultracentrifugation	745983

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Release 2023.1 (January 2023)