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EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Commentary	Reference
2.1.1.45	0.00000016	-	dUMP	mutant enzyme M190K, at 37°C	706625
2.1.1.45	0.0001	-	dUMP	mutant enzyme L198P, at 37°C	706625
2.1.1.45	0.00055	-	dUMP	mutant enzyme M190E, at 37°C	706625
2.1.1.45	0.00061	-	dUMP	mutant enzyme A191K, at 37°C	706625
2.1.1.45	0.0012	-	dUMP	wild type enzyme, at 37°C	706625
2.1.1.45	70	-	5,10-methylenetetrahydrofolate	mutant enzyme E30W, in 50 mM TES buffer, 50 mM, MgCl2, 13 mM formalin, 2 mM EDTA, 0.15 mM 2-mercaptoethanol, pH 7.4, at 25°C	720965
2.1.1.45	120	-	5,10-methylenetetrahydrofolate	presence of Mg2+, pH 7.5, 22°C	758166
2.1.1.45	120	-	dUMP	pH 7.5, 22°C	758166
2.1.1.45	180	-	dUMP	presence of Mg2+, pH 7.5, 22°C	758166
2.1.1.45	220	-	5,10-methylenetetrahydrofolate	pH 7.5, 22°C	758166

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Release 2023.1 (January 2023)