EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.2.4.2 | 0.0074 |
- |
2-oxo-5-hexenoate |
wild type enzyme, at pH 8.0 and 30°C |
762591 |
1.2.4.2 | 0.0134 |
- |
2-oxo-5-hexenoate |
mutant enzyme H298D, at pH 8.0 and 30°C |
762591 |
1.2.4.2 | 0.0165 |
- |
2-oxovalerate |
wild type enzyme, at pH 8.0 and 30°C |
762591 |
1.2.4.2 | 0.0725 |
- |
2-oxovalerate |
mutant enzyme H298D, at pH 8.0 and 30°C |
762591 |
1.2.4.2 | 0.39 |
- |
2-oxoglutarate |
mutant enzyme H298D, at pH 8.0 and 30°C |
762591 |
1.2.4.2 | 0.59 |
- |
2-oxo-5-hexenoic acid |
mutant E1o-H298D/E2o-H348Q, pH not specified in the publication, temperature not specified in the publication |
762591 |
1.2.4.2 | 1.17 |
- |
2-oxo-5-hexenoic acid |
mutant E1o-H298D/E2o-H348Y, pH not specified in the publication, temperature not specified in the publication |
762591 |
1.2.4.2 | 1.98 |
- |
2-oxo-5-hexenoic acid |
mutant E1o-H298D/E2o-H348F, pH not specified in the publication, temperature not specified in the publication |
762591 |
1.2.4.2 | 4.7 |
- |
2-oxovalerate |
mutant E1o-H298D/E2o-S333M, pH not specified in the publication, temperature not specified in the publication |
762591 |
1.2.4.2 | 6.3 |
- |
2-oxoglutarate |
pH 7.0, 25°C |
747943 |