EC Number |
Expression |
Reference |
---|
1.14.15.30 | more |
recombinant coexpression of the enzyme KshA from Mycobacterium neoaurum strain NwIB-01 and the three homologues of ketosteroid-DELTA1-dehydrogenase (KstD) from Mycobacterium neoaurum ATCC257953 in Escherichia coli strain BL21(DE3),to install a steroid production system with a broad substrate specificity. Enzyme KshA converts the KstD reaction side product 4-androstene-3,17-dione into 9alpha-hydroxyandrosta-1,4-diene-3,17-dione |
-, 743221 |
1.14.15.30 | up |
expression of kshA2 is induced in parent strain Rhodococcus erythropolis SQ1 in the presence of androsta-1,4-diene-3,17-dione or 9alpha-hydroxy-4-androstene-3,17-dione or a metabolite thereof |
-, 717119 |
1.14.15.30 | up |
in wild type Rhodococcus rhodochrous strain DSM43269, ksh1 is specifically induced by cholic acid |
-, 717735 |
1.14.15.30 | up |
in wild type Rhodococcus rhodochrous strain DSM43269, kshA2 is specifically induced by 4-androstene-3,17-dione, progesterone, and cholic acid |
-, 717735 |
1.14.15.30 | up |
in wild type Rhodococcus rhodochrous strain DSM43269, kshA3 is specifically induced by 4-androstene-3,17-dione |
-, 717735 |
1.14.15.30 | up |
in wild type Rhodococcus rhodochrous strain DSM43269, kshA4 is specifically induced by 4-androstene-3,17-dione, cholic acid, and cholesterol |
-, 717735 |
1.14.15.30 | up |
in wild type Rhodococcus rhodochrous strain DSM43269, kshA5 is specifically induced by 4-androstene-3,17-dione, progesterone, cholic acid, and cholesterol |
-, 717735 |