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EC Number	Purification (Commentary)
2.3.1.48	-
2.3.1.48	affinity column chromatography and Superdex 75 gel filtration
2.3.1.48	cells are lysed after centrifugation in 100 mM Tris-HCl buffer, pH 7.6, immunoprecipitation, affinity purification with ProFound c-Myc-Tag IP/Co-IP kit, subcellular fractionation of homogenized cells in 10 mM triethanolamine, 10 mM acetic acid, 250 mM sucrose, 1 mM EDTA, and 1 mM dithiothreitol, pH 7.4, centrifuged, membrane pellet resuspended in 5% Nycodenz and layered on top of a Nycodenz solution gradient in 10 mM HEPES, pH 7.4, fractions collected and further concentrated by ultracentrifugation
2.3.1.48	enzyme form A
2.3.1.48	enzyme form B
2.3.1.48	enzyme forms I and II
2.3.1.48	from calf
2.3.1.48	HisTrap column chromatography and Superdex 75 gel filtration
2.3.1.48	native Rtt109, in complex with the histone chaperone Vps75
2.3.1.48	native SAS complex and recombinant SAS complex, the latter with Sas2 protein being His-tagged

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Release 2023.1 (January 2023)