EC Number   |
|---|
    2.1.1.45 | - |
| 2.1.1.45 | after purification and precipitation with ammonium sulfate, wild-type and K48Q TS are frozen at -80°C |
| 2.1.1.45 | ammonium sulfate precipitation and Q-Sepharose column chromatography |
| 2.1.1.45 | Blue-Sepharose column chromatography and Q-Sepharose column chromatography |
| 2.1.1.45 | Ehrlich ascites carcinoma cells |
| 2.1.1.45 | glutathione elusion method |
| 2.1.1.45 | GST-GVE2 TS fusion protein is purified by glutathione-Sepharose beads |
| 2.1.1.45 | HiTrap Blue-Sepharose column chromatography and HiTrap Q column chromatography |
| 2.1.1.45 | L1210 cells |
| 2.1.1.45 | methotrexate affinity column chromatography |
