EC Number ![](./images/down.png) ![](./images/up.png) |
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![Show all pathways known for 1.2.1.47 Show all pathways known for 1.2.1.47](./images/citric_acid_cycle_small.png) ![Display the word map Display the word map](./images/word_maps.png) 1.2.1.47 | 14.2fold to apparent homogeneity, by hydrophobic chromatography and affinity chromatography |
![Show all pathways known for 1.2.1.47 Show all pathways known for 1.2.1.47](./images/citric_acid_cycle_small.png) ![Display the word map Display the word map](./images/word_maps.png) 1.2.1.47 | ammonium sulfate precipitation, phenyl-Toyopearl column chromatography, and 5'-AMP Sepharose column chromatography |
![Show all pathways known for 1.2.1.47 Show all pathways known for 1.2.1.47](./images/citric_acid_cycle_small.png) ![Display the word map Display the word map](./images/word_maps.png) 1.2.1.47 | recombinant FLAG-tagged enzyme TMABA-DH from HEK-293T cells by immunoaffinity chromatography |
![Show all pathways known for 1.2.1.47 Show all pathways known for 1.2.1.47](./images/citric_acid_cycle_small.png) ![Display the word map Display the word map](./images/word_maps.png) 1.2.1.47 | recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) pLysS by cobalt affinity and nickel affinity chromatography, followed by ultrafiltration and gel filtration |
![Show all pathways known for 1.2.1.47 Show all pathways known for 1.2.1.47](./images/citric_acid_cycle_small.png) ![Display the word map Display the word map](./images/word_maps.png) 1.2.1.47 | recombinant SUMO-His6-tagged ALDH9A1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the SUMO-His tag, dialysis, ultrafiltration, and gel filtration |
![Show all pathways known for 1.2.1.47 Show all pathways known for 1.2.1.47](./images/citric_acid_cycle_small.png) ![Display the word map Display the word map](./images/word_maps.png) 1.2.1.47 | using column chromatography on blue dextran-Sepharose and 5'-AMP-Sepharose |
![Show all pathways known for 1.2.1.47 Show all pathways known for 1.2.1.47](./images/citric_acid_cycle_small.png) ![Display the word map Display the word map](./images/word_maps.png) 1.2.1.47 | using column chromatography on SP-Sepharose, Red Sepharose CL-6B and Hydroxylapatite |